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Monday, October 21 • 2:30pm - 3:00pm
Poster Presentation #5- Patient-Derived Explants (PDEs) as a Powerful Ex Xivo Platform to Predict Clinical Response to Novel Anti-Cancer Drugs

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One of the leading goals of the Leicester Cancer Research Centre is to investigate novel and more relevant pre-clinical tumour models that are able to better predict patient outcomes.

Accordingly, we have established a rapidly expanding ‘Cancer Explant Facility’ whereby Patient-Derived Explants (PDEs) from freshly resected tumour specimens, are cultured ex vivo and tested with a wide panel of anti-cancer drugs including currently used therapies as well as novel drugs. This ex vivo model is a powerful platform with multiple advantages over animal models: it is patient-specific, it improves the predictivity of drug response in situ and, by accurately retaining the complexity of the tumour ex vivo, overcomes the differences between the human and animal immune systems that can lead to differential response to targeted immune-oncology therapies in animal models.

In our experimental method, drug responses are assessed using multiplexed immunofluorescence (mIF), which allows the ability to distinguish between drug effects in tumour areas versus the surrounding stroma as well as the potential contribution of the host immune system. So far, this fast and reproducible technique has been applied to different types of cancer (melanoma, lung, renal, breast and CRC liver metastasis) to analyse the effect of anti-cancer agents on cell viability, proliferation and immune cell mobility. Finally, by combining this platform with gene expression, genetic and epigenetic analysis, we also aim to identify novel biomarkers that correlate with, and predict, the response of cancers to anti-cancer drugs as well as their toxicity in preclinical settings, prior to clinical testing.

Speakers
GV

Giuditta Viticchie, PhD

Post Doctoral Research Associate, University of Leicester
2008: Master’s degree in Chemistry at the University of Rome “Tor Vergata”, Italy.2012: PhD in Biochemistry and Molecular Biology at the University of Rome “Tor Vergata”, Italy2012-2013: Post Doctoral Fellow at the MRC Toxicology Unit Leicester, UK, with Italian fellowship... Read More →


Monday October 21, 2019 2:30pm - 3:00pm
Sherry Coutu Seminar Suite Foyer