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Tuesday, October 22 • 10:50am - 11:10am
Advances in Imaging and Analysis- Imaging Rapid Cell Shape Transitions and Volume Control in 3D

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Imaging rapid cell shape transitions and volume control in 3D
Regulation of cell shape in 3D space is essential during human development, and misregulation of cell shape is central to human disease states such as metastatic cancer. We are using 3D high content imaging to understand how healthy and diseased cells regulate their 3D shape and volume.

Approach: Oblique plane microscopy for 3D imaging
To make measurements in 3D we are taking advantage of recently developed Oblique Plane Microscopy (OPM). OPM imaging combines the benefits of light sheet microscopy - high speed 3D imaging, and low phototoxicity - with the advantages of conventional microscopy such as simple mounting techniques and high throughput sampling.

i) Cell shape transitions
To understand the molecular networks controlling 3D shape transitions in melanoma, we are live imaging cells embedded in collagen matrices. We are using this system to establish baseline shape dynamics, as well as shape transitions when cytoskeletal regulators are inhibited or when cells are in the presence of clinical drugs.

ii) Control of cell volume
Volume control is key to cell function because the volume of a cell influences the scale, duration and dynamics of all biochemical processes within. We have been using volumetric imaging by OPM in immortalised epithelial cells to understand how cell geometry is maintained throughout each stage of the cell cycle, and across cell generations. We anticipate these discoveries can form the basis of new strategies to target size control checkpoints in the treatment of cancers.

avatar for Lucas Dent

Lucas Dent

Postdoctoral Fellow, Institute of Cancer Research
My research aims to understand the signalling networks controlling cell shape and adhesion during normal development, and how these are altered in disease. To do this, my approach is to combine Drosophila (fruit fly) genetics and mammalian (human) systems to reveal the broad principles... Read More →


Tuesday October 22, 2019 10:50am - 11:10am BST
Wellcome Auditorium