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Monday, October 21 • 2:30pm - 3:00pm
Poster Presentation #7- High-Content Assay for Morphological Characterization of 3D Neuronal Networks in a Microfluidic Platform

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Establishment of physiologically-relevant in vitro models is crucial to further understanding of the mechanisms of neurological diseases as well as targeted drug development. While iPSC-derived neurons show great promise for compound screening and disease modeling, use of three-dimensional (3D) cultures is emerging as a valid approach for neuronal cell based assay development. 3D cultures are recognized as more closely recapitulating aspects of the human tissues including the architecture, cell organization, as well as cell-cell and cell-matrix interactions.

This poster presents the development of a high-throughput 3D neurite outgrowth assay using iPSC-derived neurons developed in the microfluidic OrganoPlate® platform, with the goal of establishing 3D models for neurodegenerative diseases and neurotoxicology screens. 3D cultures were treated with known neurotoxic compounds and the morphology and viability of neurons were assessed. The OrganoPlate® from MIMETAS is a high-throughput platform that combines the most recent advances in 3D cell culture, Phaseguides™ and microfluidics. The OrganoPlate contains 96 tissue chips suitable for long term culture of live cells, is amenable for screening purposes, and is compatible with standard laboratory equipment or automated systems, like the ImageXpress® Micro Confocal High-Content Imaging System.

Speakers
AB

Andy Bashford

Application Scientist, Molecular Devices
Andy works as a Field Applications Scientist for Molecular Devices covering the whole portfolio of imaging solutions.Prior to this he worked as a development scientist for the European Collection of Authenticated Cell Cultures (ECACC) where he was responsible for developing assays... Read More →


Monday October 21, 2019 2:30pm - 3:00pm
Sherry Coutu Seminar Suite Foyer