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Tuesday, October 22 • 1:30pm - 2:00pm
Poster Presentation #20-AI-driven Optimization of Multidrug Combinations in Heterotypic 3D Human Co-cultures

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Heterotypic three-dimensional (3D) short-term human cellular models were developed based on the histopatological patient tissues to predict the efficacy of new (combined) treatment regimens for treatment of various stages of human colorectal carcinoma (CRC). These spheroidal callular co-cultures included human CRC cell lines of various genetics and stage co-cultured with human endothelial cells and fibroblasts.

The exposure of those spheroids with several low-dose three- or four-drug combinations, designed using artificial-intelligence-based method allowed efficient optimization of the most effective treatment strategy.

The optimized synergistic cell type-specific drug combinations induced cell metabolic activity inhibition, that indirectly corresponding to cell viability inhibition, by up to 90%. Moreover, the activity of those optimized drug combinations was often more potent in 3D models as compared to simple 2D cell cultures and facilitated further translation to more complex in vivo model.

Summarizing, our technology represents an innovative step to obtain enhanced accuracy of the activity of cell type-specific treatments. Our multicellular spheroids resemble in many aspects solid tumors and serve as a promising model to mimic the in vivo situation.

Speakers
PN

Patrycja NowakSliwinska, Ph.D., D.Sc.

Professor, Institute of Pharmaceutical Sciences of Western Switzerland
She performed her post-doctoral work in the Medical Photonics Group at the Swiss Federal Institute of Technology (EPFL) in Lausanne, Switzerland, and in the Angiogenesis Laboratory at the VU University Medical Center in Amsterdam, The Netherlands. She obtained the certificate of advanced... Read More →


Tuesday October 22, 2019 1:30pm - 2:00pm
Sherry Coutu Seminar Suite Foyer