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Monday, October 21 • 2:30pm - 3:00pm
Poster Presentation #3- Magnetic Electrospun Microfibre Scaffold Assemblies: Examples of there use for Cell-Based Screening Applications

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In an effort to create a more physiological three-dimensional (3-D) environment for cell growth while maintaining the logistics of well plate screening for drug discovery purposes, we have develop a 3-D micro-scaffold platform from electrospun material used in conjunction with well plates for higher throughput screening. We have engineered electrospun material to form micro-scaffold islands of cells. Cells grow on, around and into the material, forming a micro-island of adherent cells that are effectively 3-D in solution. The incorporation of iron nano-particles into the fibres during manufacture results in micro-scaffold islands that can be physically manipulated using electro-magnetism. We can culture cells on micro-scaffolds in culture vessels in media within the incubator in addition to moving them within wells using arrays of neodymium magnets, attracting scaffolds to the edges of wells to prevent damage during media replacement or align scaffolds in the centre of wells for imaging purposes. Using sheathed electromagnetic tips we can move micro-scaffolds from well to well with no adverse effects to cells. In addition we have developed a liquid handling device capable of identifying the location of scaffolds in a petri dish then performing a ˜pick and place™ procedure putting them into user defined wells of a plates. Furthermore, we have incorporated fluorescent dyes or quantum dots into the fibres such that fibres can be visualised using fluorescence microscopy, while quantum dots can be used as a barcode to distinguish between two cell populations on different scaffolds within the same well. Cells can be transfected while cultured on micro-scaffolds using either lipofection or electroporation. Recombinant cells can be cryo-preserved on micro-scaffolds. We will show data of exemplar assays using recombinant, primary and differentiated stem cell assays on micro-scaffolds. We have used this approach to examine a number of luminescence and fluorescence readouts in recombinant cell lines and have differentiated iPSC™s to cortical neurones in 3-D on micro-scaffolds to characterise then use imaging as a readout. Our data suggests that this micro-scaffold approach may open up new opportunities for both recombinant cell based screening and differentiated stem cell assays using single and co-cultures in a well plate-based format more amenable to higher throughputs with very little manual intervention.

Speakers
GA

Gary Allenby, Ph.D.

Chief Scientific Officer, Aurelia Bioscience
PhD in reproductive tox, worked in pre-clinical pharma for 25 years in Hit ID, Hits to Lead, Target Biology sections developing cell based assays for compound screening. Founding entrepreneur of Aurelia Bioscience in 2012, delivering tailored assay development for compounds pharmacological... Read More →


Monday October 21, 2019 2:30pm - 3:00pm
Sherry Coutu Seminar Suite Foyer