Loading…
Go to symposium website → www.slas.org/HighContentAnalysis
Tuesday, October 22 • 1:30pm - 2:00pm
Poster Presentation #13-A Functional Study of the Endomembrane System in Mammalian Cells Growing in a 3D Environment

Sign up or log in to save this to your schedule and see who's attending!

The human body is a complex assembly of tissues and organs, each of which is made of different types of cells. Specialisation of function is achieved via compartmentalisation within each cell, as well as defined communication networks that exist between endomembrane compartments. The endomembrane system is essential for the synthesis and distribution of proteins, lipids and carbohydrates, and as such needs to be tightly regulated. One protein family of master regulators of the endomembrane system is the Rab small GTPases. Rab proteins govern the communication between organelles, via the GDP-GTP cycle and by exhibiting specific membrane localisation patterns. To date, their distribution and function have mostly been investigated in conventional two-dimensional in vitro cell models, however three-dimensional cell models, so far applied to investigate cancer and drug development, offer new possibilities. In this work, a functional study is being used to understand the localisation and role of Rab proteins in the endomembrane system in a three-dimensional environment. For this, a three-dimensional cell model (spheroids), using HeLa Kyoto cells grown on micropatterned plates, has been developed. This approach allows highly uniform spheroids to be grown in a tightly controlled manner. In order to localise various Rab proteins, stably expressing cell lines expressing GFP-tagged Rab proteins have been produced and grown as spheroids. Automated high-content screening microscopy is now being used to quantitatively describe the localisation and function of Rab proteins in this new cell system, and in turn, compare the results to existing data coming from classical two-dimensional cell models.

Speakers
MM

Margaritha Mysior, B.Sc., M.Sc.

PhD candidate, University College Dublin
I absolved a Bachelor (2012-2015) and Master (2015-2017) degree program at the University of Applied Science Gelsenkirchen, Recklinghausen and Bocholt. During my Master thesis (October 2016 - August 2017) I characterised five unidentified regulators of the retrograde transport pathway... Read More →


Tuesday October 22, 2019 1:30pm - 2:00pm
Sherry Coutu Seminar Suite Foyer