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Tuesday, October 22 • 1:30pm - 2:00pm
Poster Presentation #19- Fully Automated and Scalable 3D-Baed Toxicity Testing in Human Neural Microtissues

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Recent advances in stem cell technology have led to the development of three-dimensional (3D) culture systems, which have fueled hopes to bring about the next generation of more physiologically relevant high throughput screens (HTS). However, current protocols yield either complex but highly heterogeneous aggregates (“organoids”) or 3D structures with less physiological relevance (“spheroids”). Here, we present a fully scalable, HTS-compatible workflow for the automated generation, maintenance, and high content analysis of human midbrain-like automated microtissues (mANTs) in a standard SBS 96-well format. The resulting microtissues possess a highly homogeneous morphology, size, global gene expression, cellular composition, and internal organization. They present significant features of the developing human midbrain and display spontaneous aggregate-wide synchronized neural activity. By automating the entire workflow from generation to analysis, we provide essential intra- and inter-batch reproducibility as demonstrated via RNA sequencing and quantitative whole mount high content imaging. Dose response toxicity testing showcases our ability to quantify drug effects on single cells within a complex 3D environment using mANTs. In conclusion, our workflow has the potential to form the basis for 3D-tissue-based screening in a variety of applications including toxicology studies, disease modelling, and drug development.
 

Speakers
HR

Henrik Renner, Msc.

PhD Student, Max Planck Institute for molecular Biomedicine
- PhD student in the lab of Hans Schöler at the MPI in Münster (since 2016), Germany (creating 3D HTS tissue models for midbrain)- Msc. Karolinska Institute (2016)- Bsc. University of Göttingen (2014)


Tuesday October 22, 2019 1:30pm - 2:00pm
Sherry Coutu Seminar Suite Foyer